Rigor Buffer

4L final volume
75mM KCl(f.w. 74.56) 22.4g
1mM MgCl2.6H2O(f.w. 203.31) 4ml
10mM K phosphate(0.1M PB) 40ml
2mM EGTA 40ml
1g/ml Penicillin 4ml
10% Triton 20ml

10mM PMSF(0.17g/100ml-95%alcohol) 80ml before use