SAMPO FT-1010 Function List

Func. 1: set date/time
Func. 2: set TTI name & No.
Func. 3: message confirmation (Go/Yes/No)
Func. 42: select footline print (Yes/No)
Func. 43: select speed 4800 bps (Yes/No)
Func. 44: select memory clear (Yes/No)
Func. 45: select dial mode (0:tone/1:10 pps/2:20 pps)
Func. 46: select extended dial (Yes/No)
Func. 48: select paper save (Yes/No)
Func. 49: auto journal list (Yes/No)
Func. 5: set Fax/Tel No. & name (2x27 sets)
Func. 71: select poll (Yes/No)
Func. 72: set poll password (0000-9999)
Func. 73: set answer ring #(01-99)
Func. 74: set redial times #(1-9)
Func. 75: set speaker volume (0-3)
Func. 81: journal list
Func. 83: stored Fax/Tel No. & name list
Func. 85: system configuration list
Func. 86: communication error message list
Func. 87: printer test & pattern list
Func. 88: Function list

Phosphate Buffered Saline (PBS)

10X PBS pH7.4
Final Conc. F.W. 1 L 2L
NaCl 1.37 M 58.44 80.06 g 160.13 g
KCl 2.7 mM 74.55 0.2 g 0.4 g
Na2HPO4 101.4 mM 177.99(2H2O) 18.5 g 36.1 g
KH2PO4 17.6 mM 136.09 2.4 g 4.79 g

Rigor Buffer

4L final volume
75mM KCl(f.w. 74.56) 22.4g
1mM MgCl2.6H2O(f.w. 203.31) 4ml
10mM K phosphate(0.1M PB) 40ml
2mM EGTA 40ml
1g/ml Penicillin 4ml
10% Triton 20ml

10mM PMSF(0.17g/100ml-95%alcohol) 80ml before use

Biuret Assay

Reagent
per liter final volome
6g sodium potassium tartrate (NaKC4H4O6.4H2O)(f.w. 282.22)
1.5g copper sulfate (CuSO4.5H2O)(f.w.249.68)
all dissolved(500ml) in order in 30g/300ml NaOH(f.w. 40.0) before bringing to fianl volume.
Discard if a black precipitate forms.

Assay
1. Warm up the spectrophotometer 10 min. before use.
2. Prepare standards from bovine serim albumin (BSA).
3. Using 4ml biuret reagent to 1 ml sample a recommended range is 0.5 to 20 mg protein.(If possible, dilute unknowns to an estimated 1 to 10 mg/ml with buffer.)
4. Use 1ml sample per assay tube and add 4ml Biuret reagen to each tube, vortex immediately.
5. let stand 20 min.
6. Read at 540nm.

BSA 10mg/ml standard curve exsample
1(0.049),2(0.097),4(0.186),8(0.374),10(0.459) with rigor buffer.

Phosphate Buffer recipes

0.2M NaH2PO4 0.2M Na2HPO4 pH
NaH2PO4.H2O(f.w. 137.99) 2.76g/100ml Na2HPO4(f.w. 141.96) 2.84g/100ml
93.5 6.5 5.7
92.0 8.0 5.8
90.0 10.0 5.9
87.7 12.3 6.0
85.0 15.0 6.1
81.5 18.5 6.2
77.5 22.2 6.3
73.5 26.5 6.4
68.5 31.5 6.5
62.5 37.5 6.6
56.5 43.5 6.7
51.0 49.0 6.8
45.0 55.0 6.9
39.0 61.0 7.0
33.0 67.0 7.1
28.0 72.0 7.2
23.0 77.0 7.3
19.0 81.0 7.4
16.0 84.0 7.5
13.0 87.0 7.6
10.5 89.5 7.7
8.5 91.5 7.8
7.0 93.0 7.9
5.3 94.7 8.0
ml mixed to 200ml with ddw (0.1M K phosphate)